Meeting Banner
Abstract #2170

Classification of Neural Activity Patterns Observed by PhMRI Upon Pharmacological Interventions in Rats

Basil Knnecke1, Andreas Bruns1, Eric Prinssen1, Jean-Luc Moreau1, Joseph G. Wettstein1, Markus von Kienlin1, Cline Risterucci1

1CNS Research, F. Hoffmann-La Roche Ltd, Basel, Switzerland

Preclinical pharmacological fMRI (phMRI) has been successfully used to elucidate the effects of psychoactive drugs on regional cerebral activity, but comparative meta-analyses of study ensembles are largely missing. The present work provides such a systematic and quantitative analysis of perfusion-based phMRI data obtained upon interventions with an extensive set of neuroactive reference compounds impacting on the domains of anxiety and depression. Neural activity patterns detected by phMRI were subjected to principal component analysis in order to yield specific signatures for the drugs modes-of-action and objective measures of dose-effect and treatment duration-effect relationships.

Keywords

action activation activity acute administration aimed anatomically animals anxiety arterial attention away background basil belonging best blood bluish brain breathing challenges classical classification classifying clearly close closing clustered coil combinations component components compounds comprised condition consistent continuous control cortex cross defined delay demonstrating dependent depression described differential dimensional distinct documented domains dose doses dosing drug drugs duration elicited equipped excerpt extensive feature fledged flow freely full generally gray greenish head hence impacting induced instrument interpretation interventions inverted investigated investigation kept known matrix measures medial modes moreover moved naive network neural nodes notion novel nucleus objective opposing options orthogonal outcomes patterns perfusion pharmacological post principal proximity quantitative quantitatively rare rationale rats receive reddish related relationships remained repeated respective response revealed robustness scores segregation selected selective separated serve signatures slice space spanning spin stance strongly structures studies subjected successful supported supports symbols systematic systems targeting transmit treatment upon vehicle versus virtually yield yielded