Meeting Banner
Abstract #1694

Metabolism of Hyperpolarized [1-13C]Pyruvate in the Isolated Perfused Rat Lung an Ischemia Study

Stephen Kadlecek1, Benjamin Pullinger1, Kiarash Emami1, Harrilla Profka1, Masaru Ishii2, Rahim Rizi1

1Radiology, University of Pennsylvania, Philadelphia, PA, United States; 2Otolaryngology-Head and Neck Surgery, Johns Hopkins University, Baltimore, MD, United States

Difficulties with NMR studies of lung arising from low tissue density and air-tissue interfaces can be partially overcome using 13C-DNP NMR. This experiment studies the evolution of the [1-13C] lactate signal in isolated, perfused rat lungs after injection of pyruvate under normoxic and ischemic conditions in order to understand changes in lung oxidative state, central to ischemia-reperfusion injury, transplantation, and metabolic changes in cancer.

Abstract PDF Poster PDF

Keywords

accumulation active additional administration albumin allowed analyzed approximates arising artery bovine broadening buffer care chemical clamped cohorts collapse collected combination comparable concentration conditions confirm constant contained course decreased density despite destructively determined difficult difficulties direct dissolve encountered energy excised exhalation experiment extracted extracts fitting five flow freeze frozen global glucose heated highly identical immediately in vivo individual inflation initial injected injection injury integral integrals interfaces isolated johns kept label lactate least loss lung lungs magnet metabolic metabolism metabolites minutes near neutralization nominal normalized normalizing onset organs outliers overall oxidative oxygenating parameterized part partially peak peaks perfused perfusion phosphate physiological placed pools post prepared probe problem production protect proton pulmonary rats recovered recovery reduction regional reported represented resembled residual retention routine sample serum several significantly solid solution sooner spectra spectral spectroscopy started steady stopping storage studied studies subsequent suggesting system tail tend tissue tissues trachea transplantation transversely tumor typical unlikely upfield vein viability yielded