Meeting Banner
Abstract #1683

Compartmentation of MCF-7 Tumour Cell Metabolites Characterised by Hyperpolarised 13C Diffusion-Weighted Spectroscopy

Franz Schilling1, 2, Stephan Dwel1, 2, Markus Durst1, 3, Ulrich Kllisch1, 3, Jan Henrik Ardenkjaer-Larsen4, Pedro A. Gmez Damin3, Markus Schwaiger5, Rolf F. Schulte3, Steffen J. Glaser2, Axel Haase1, Angela Otto1, Marion I. Menzel3

1Zentralinstitut fr Medizintechnik, Technische Universitt Mnchen, Garching, Germany; 2Department of Chemistry, Technische Universitt Mnchen, Garching, Germany; 3GE Global Research, Munich, Germany; 4GE Healthcare, Copenhagen, Denmark; 5Department of Nuclear Medicine, Technische Universitt Mnchen, Munich, Germany

Understanding tumour metabolism is a central issue in diagnosis of tumours. Using magnetic resonance spectroscopy, hyperpolarized [1-13C]pyruvate and its metabolites can be detected to characterise the stage of a tumour. The signal of each metabolite emanates from both extra- and intracellular compartments. In this work, we show for the first time, using real-time direct detection, that diffusion coefficients of [1-13C]pyruvate and its metabolites in tumour cell spheroids can be determined. We foresee many new applications of hyperpolarized 13C diffusion-weighted spectroscopy e.g. to separate intra- and extracellular compartments of tumours, and to separate metabolic conversion from perfusion by large diffusion weightings.

Abstract PDF

Keywords

acknowledges acquisition agents amplitudes apparent around bike breast cancer caused cell cells cellular chemistry clearly coefficient coefficients collected compartments concentration consists containing continuing conversion corrected correction correlation currently damping dashed decay decreased decreasing described detection determine determined diagnosis differentiate diffusion direct disruption dissolved distance durst efforts emanates equation excitation exponential exposing expression extra feasible final fitted fitting focus fold fonds funded future glass global gradient grant half human hydrate in vivo incrementally indicates indicating indirect injected injection inner instruments intracellular issue kinetic lactate largest leakage like limitations loop made magnet many markedly measured medicine membrane metabolic metabolism metabolite metabolites million model modified nuclear overcome oxford partly perfusion permanent polarizer pulsed rapidly real reduced reducing repeated response restricted sample schilling separate separated serving side significantly slow slower solution spectra spectroscopic spectroscopy spheroids spin starting steam structure suited susceptibility system taken tanner thank throughout transport transported treatment tube understanding uptake varied vary weightings whole width