Meeting Banner
Abstract #0497

In Vivo Comparison of Total & Hyperpolarized Lactate Levels Assessed by Localized 1H MRS & Hyperpolarized 13C MRSI in Glioblastoma Models at 14.1Tesla

MAGNA25Myriam Marianne Chaumeil1, Gerd Melkus1, Sarah Woods1, Robert M. Danforth1, Alessia Lodi1, Aaron Robinson2, Joanna J. Philips2, 3, Sabrina M. Ronen1

1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States; 3Pathology, University of California, San Francisco, San Francisco, CA, United States Localized 1H MRS and hyperpolarized (HP) 13C MRSI were performed in vivo at 14.1Tesla to assess total and HP lactate levels, respectively, in two orthotopic models of glioblastomas with significantly different intracellular lactate levels: U87IDHmut[Lac]>U87IDHwt[Lac]. In line with the intracellular levels, in vivo 1H lactate levels were significantly higher in U87IDHmut. In contrast, HP lactate-to-noise and lactate-to-pyruvate ratios from the same tumor voxel were not significantly different between the cell lines. This discrepancy confirms that HP 13C MRSI and 1H MRS provide complementary information, the 1H-detected lactate level not being a dominant factor in the detected HP lactate production.

 

Abstract PDF Presentation Video

Keywords

acid acknowledgments acquisition activities activity affected amount anatomical anesthetized animals assess assessed assessment axial black blue bottom brain buffer catheter cell cells clearly coil combination combined comparing complementary conclude confirms conjunction containing content contrast coronal course culture cultured delivery depend detected determined dissolved distinct dynamic elevated evaluate evaluation even exogenous experiment extraction extracts fellowship field form furthermore glucose gradients grant grid hand highly in vivo injected injection inside instruments integrals intra intracellular inverted lactate length lipid lipids localization localized long matrix measured mechanisms metabolic metabolism mice millipede models modulations mutant mutation neurological noise normalized nuclear often optimized package particular pathology peak polarization polarizer pools positioned post press previously processed processing produced production prove questioned radical radiology rapidly real reflects report reprogramming resolution respectively senate separate several shimming short significantly slices software solution spectra spectroscopy spin square studies supplemented suppression suspension system tail though transporter tumor tumors types useful variable vein whether wide woods