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Abstract #0063

Diffusion Investigation of Intramyocellular Lipid Droplet Changes in Skeletal Muscle Under Fasting Condition

SUMMA25Peng Cao1, 2, Zhongwei Qiao1, 2, Anna M. Wang1, 2, Shujuan Fan1, 2, Victor B. Xie1, 2, Jian Yang1, 3, Ed X. Wu1, 2

1Laboratory of Biomedical Imaging and Signal Processing, The University of Hong Kong, Hong Kong SAR, China; 2Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong SAR, China; 3Medical Imaging Center of the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an, Shanxi Province, China

Biophysical properties of intramyocellular lipid (IMCL) such as droplet size may reflect the balance between droplet synthesis and degradation. In this study, we hypothesized that characterizing the restricted diffusion of IMCL protons could serve as a sensitive marker for monitoring IMCL droplet dynamics during metabolic intervention/abnormality. Our experimental results demonstrated that in vivo IMCL diffusion characteristics are sensitive to metabolic manipulation by fasting. During the 60-hr fasting, IMCL level increased but exhibited more restricted diffusion, largely in agreement with our histological observation of more droplets but of smaller sizes. Such IMCL diffusion characterization may serve as a sensitive marker to probe the IMCL droplet dynamics in vivo.

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Keywords

abnormality adding adult aimed alone alter alters animal animals axis basic biophysical caused cell cells characterization china column computed condition confounding considered control core correlation critical cycle decrease decreased degradation dependent device diffusion disorders divided dots drastic drastically droplet droplets dynamics employed energy equipped ester examination examine examined excised exist fasted fasting feeding fitting fixation form generally gradients highly histology hong importantly improved improving in vivo increasingly indicated indicating insufficient insulin intervention interventions intracellular laboratory lead lipid long made marker materials medicine metabolic metabolism micron mixture motions much muscle nature negative obesity package pair paralyzer people physiological positioned preliminary preparation processes processing protocol proton protons quantification rats recognized reduced reflect related relatively relevant reported republic reservoir resistant respectively restricted role room samples scanner scattered school sensitive sensitivity serve several shape significantly since skeletal smaller spectra spectral spectrum spherical staining steam stimulated student substantially suggesting surface synthesis system tone treatment typical unpaired width yang