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Abstract #4058

Effect of Mesenchymal Stem Cells on the Vascularization of the Artificial Cavity Used as a Site for Islet Transplantation

Daniel Jirak1, Jan Kriz1, Peter Girman1, Eva Vodraskova1, Klara Zacharovova1, Eva Dovolilova1, Vit Herynek1, Frantisek Saudek1, Milan Hajek1

1Institute for Clinical and Experimental Medicine, Prague, Czech Republic

To improve transplantation outcomes, artificial sites for islet transplantation are tested. The aim of our study was to evaluate the effect of mesenchymal stem cells on the blood supply of the artificial cavities for islet transplantation created subcutaneously or within the greater omentum in a preclinical rat model. Neovascularization within biocompatible devices with/without mesenchymal stem cells was assessed during the four weeks following implantation by dynamic contrastenhanced magnetic resonance imaging. Our results indicate that artificial device containing the mesenchymal stem cells provides superior blood supply for the transplanted cells and longer optimal time for cell transplantation.

Keywords

ability achieved actual adequately adipose administration advance adverse agent analyzed animals another application artifacts artificial artificially assessed attention audience basal better blood brown cause cavities cavity cell cells characterized clinical coil community composite confirmed connective considerable considered containing contrast controls covered created cycle cycles days delay destroyed detected detection device devices differentiate differentiation dimensional drug dynamic endothelial enhanced every evolution experiment experimental feeding female flow functional gallant gradient graft great greater health hours identification immunoassay impairment implantation implanted indicate induced influence infusion injected injection intensity islet islets isolated kidney least locations longer manually maximal measured medicine medium meshes minimize ministry model need normalized optimal outcome owing pancreatic penetrated penetration peter polymer polymeric porous portal production project rats relevance repetitions republic researcher resolution resonator rounded scaffolds scanned scientific served site sites stem storm subcutaneous subcutaneously summarized supply support supportive surface tail target tissue transplantation transplanted type variability vein verified verify week weeks