Meeting Banner
Abstract #1972

Cerebral Metabolic Response to Methylene Blue: A 31P Magnetization Transfer Study at 11.7T

Andrew Bresnen1, Fang Du1, Geoffrey Clarke2, Timothy O. Duong3

1Research Imaging Institute, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States; 2Radiological Sciences, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States; 3UT Health Science Center at San Antonio, San Antonio, TX, United States

Methylene blue (MB) has unique energy-enhancing and antioxidant properties. MB shows remarkable therapeutic effects in a number of neurological disorders, including Alzheimer's and Parkinson's diseases and traumatic and ischemic brain injury. In vitro studies have demonstrated that MB enhances mitochondrial ATP production via oxidative phoysphorylation, however the effects of MB on in vivo metabolites and metabolic rates remain unknown. In this study, we implemented 31P NMR based on the Four Angle Saturation Transfer method for rapid in vivo measurements of the effects of MB on relative concentrations of ATP, phosphocreatine and inorganic phosphorus and the forward creatine kinase (CK) rate.

Abstract PDF Poster PDF

Keywords

ability accurate achieved acquisition acts adiabatic aging allows almost anesthesia animals antioxidant applications audience auto availability balance bleed blue brain brains breath buffer bypassing cell cerebral chain characterized chem coil complex concentrations consisting continuously course cycler decreased delivery described diseases disorders disturb dose dosing dual electron electrons element elevated elevation energetic energy enhancing every exclusively fang fast firmly forward future health hyperbolic immediately implies important in vivo injury insights involved magnet magnetization maintained maintaining male measure meet membranes metabolic metabolite metabolites methylene moved negligible neurological normalized optimize oxidative oxidizing oxygen phosphorus physiological placed positioning positive post potential preserving previously prior produce production property pulses radiological rapid rats reaction reactive rectal redirect reduces regimens relatively relies relying remain remained response returned role saturation science sciences shimming significantly site species spectra stable stroke studied studies suggests supports surface synthesis tail target temperature therapeutic thereby throughout tissue transfer transport traumatic treatment tuned typical understanding vitro whole