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Abstract #1907

A Dual Fluorescent/Paramagnetic Chemical Exchange-Based MRI Probe for Cell Death Imaging

Jonatan A. Snir1, Mojmr Such2, 3, Alex X. Li4, Robert H.E Hudson5, Stephen H. Pasternak6, 7, Robert Bartha8

1Department of Medical Biophysics, Robarts Research Institute, London, Ontario, Canada; 2Department of Chemistry, Western University, London, Ontario, Canada; 3Imaging Research Laboratories, Robarts Research Institute, London, On, Canada; 4Imaging Research Laboratories, Robarts Research Institute, London, Ontario, Canada; 5Department of Chemistry, Western University, London, On, Canada; 6Department of Clinical Neurological Sciences, Robarts Research Institute, London, Ontario, Canada; 7Department of Physiology and Pharmacology, Schulich School of Medicine, Western University, London, Ontario, Canada; 8Imaging Research Laboratories, Robarts Research Institute, London, Onta, Canada

Programmed cell death (apoptosis) is an important feature for studies of cancer therapy as well as ischemia and neurological pathologies. Recently, we have synthesized a novel MRI and fluorescent contrast agent containing a cleavage site specific to Caspase 3. Prostate cancer cells were either irradiated with ultraviolet radiation or maintained without insult. 24 hour incubation with our contrast agent permitted differentiation of irradiated versus control cells with both confocal microscopy and PARACEST MRI. This work provides a proof-of-principle of PARACEST imaging for the detection of apoptotic cell population following ionizing radiation.

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