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Abstract #1906

Evaluation of a TGase-Responsive PARACEST MRI Contrast Agent: The Influence of Conformations and Non-Covalent Adducts

Dina V. Hingorani1, Edward T. Randtke1, Marty Pagel2

1Chemistry and Biochemistry, University of Arizona, Tucson, AZ, United States; 2Biomedical Engineering, University of Arizona, Tucson, AZ, United States

We have characterized the CEST effect of a TGase-responsive PARACEST MRI contrast agent that is conjugated to a protein and hydrophilic peptides that include arginine. The appearance of CEST at -11 ppm differs from the appearance of CEST at +4, +11, and +22 ppm after conjugation of the agent a hydrophobic ZQR peptide, which demonstrates outstanding specificity of CEST for different molecular conformations, which may be exploited to design responsive CEST agents. CEST at -11 ppm before TGase-catalyzed conjugation indicated the formation of a non-covalent supramolecular adduct, which may be further exploited to design responsive CEST agents.

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Keywords

acid activity adding adduct adducts agent agents albumin analyses appear appearance applied arginine arising assigned behavior biochemistry biomedical bond buffer carried catalysis catalyzed caused chain characterize chelate chelation chemical chemistry confirmed conformations conjugate conjugation construct contain containing contrast covalent creation demonstration design detect differs engineering enzyme evaluation exchange exploited fitting formation frequencies furthermore heights hours hydrophilic hydrophobic identify improve increments incubated influence intermediates investigated investigating listed magnitude mass measure media mediated microbial mixture molecular outstanding paramagnetic peak peptide peptides power preparation previous prior product products protein proximity pulse reactant reactants reaction reactions recombinant replacing report reported responsive sample saturation series side solution sought spanning specificity spectra spectrometry spectroscopy spectrum suggests synthesis synthesized units utility validate water weak