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Abstract #1874

Combined Molecular MRI and Immuno-Spin-Trapping for in vivo Detection of Membrane-Bound Radicals in Mouse GL261 Gliomas

Rheal A. Towner1, Nataliya Smith1, Debra Saunders1, Patricia Coutinho De Souza1, Leah Henry1, Florea Lupu2, Robert Silasi-Mansat2, Marilyn I. Ehrenshaft3, Ronald P. Mason3, Sandra E. Gomez-Mejiba4, Dario C. Ramirez4

1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2Cariovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 3Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States; 4Laboratory of Experimental Medicine & Therapeutics, National University of San Luis, San Luis, Argentina

Free radicals associated with oxidative stress play a major role in cancer. By combining immuno-spin-trapping (IST) and targeted molecular magnetic resonance imaging (mMRI), we detected in vivo levels of membrane-bound radicals (MBR) within tumors of GL261 glioma-bearing mice. The spin trapping agent DMPO (5,5-dimethyl pyrroline N-oxide) traps MBR which can be detected in vivo with a targeted molecular MRI probe that detects DMPO-trapped-MBR. This is the first report regarding the detection of in vivo levels of MBR from a glioma model. This novel non-invasive method can be applied to investigate free radical mechanisms in the pathogenesis of various cancers.

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Keywords

acquisition acute adducts administered administration advanced advantage agent albumin anesthesia animals anti antibodies antibody anticipated applied assessment bearing biology biotin bound bovine brain brains cancer cell cells cellular city coil combination components computed confirmed consider contrast control daily days decrease detect detected detection differentiation disease either elevated environmental equipment events every evolution extent fitting fluorescence form foundation free funding growth health henry heterogeneous histogram hours implantation in vivo inactivation indicated indicating injected intensity intervals invasive laboratory lipid major maps mason measured mediated medical medicine membrane mice microscopy model models molecular mouse national nonlinear note organs overlaid oxidation oxidative oxidatively oxide oxygen park pathological peroxide pixel post prepared previously prior probe procedure protein protocol radical radicals rapid reconstructed regional repetition resolution response restrained rodent saline samples serum smith spatial species specified spin starting sustained synthesis taken targeted targeting technologies threshold timing tissue tissues towards towner transduction transverse trapping treated triangle tumor understanding uptake vitro