Meeting Banner
Abstract #1387

Development of Cardiac MRI for Studying Zebrafish Models of Cardiovascular Disease

Gavin D. Merrifield1, Jim Mullin2, Carl S. Tucker3, Martin A. Denvir3, William M. Holmes2

1School of Clinical Sciences and Community Health, University of Edinburgh, Edinburgh, Scotland, United Kingdom; 2Glasgow experimental MRI Centre, University of Glasgow, Glasgow, Scotland, United Kingdom; 3Cardiovascular Sciences, University of Edinburgh, Edinburgh, Scotland, United Kingdom

The zebrafish has emerged as an excellent model for cardiovascular research, with many researchers being attracted by the increasing availability of molecular, genetic and physiological research tools. However, unlike with mammalian models, live imaging of heart function remains challenging in the adult zebrafish1. Unlike embryos (<5day), the skin of adult zebrafish is non-transparent, i.e. pigmented and scaled, thereby precluding optical methods. We have explored the possibility of using MRI for in-vivo cardiac imaging of adult zebrafish, by implementing a retrospective self-gated cardiac sequence.

Abstract PDF

Keywords

abdomen ability acquisition addition adult allowing application array assign attracted availability beat beating brain cadavers cardiac cardiovascular cell challenging channel cine circulation clinical coil community continues corresponds costs critical cycle death development difficulty disease eggs embryos emerged employ employing equipped ever excellent experimental explored extremely female fish flow function future gated gating genetic gradients growth health heart highlighted immediately implementing important impractical in vivo increasing injury kingdom live maintenance mammalian many martin matrix micro minutes model models molecular muscle navigator nontransparent note optical overcome particularly period physiological pigmented pilot possibility precluding prove rapid regard regenerate remains remarkable repetitions researchers resolution respiratory retrospective retrospectively scaled scanned school sciences self significantly skin slice space studies system thereby tools tucker unlike