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Abstract #0804

Interplay of Choline Metabolites and Genes in Patient-Derived Breast Cancer Xenografts

Maria T. Grinde1, 2, Saurabh S. Gorad1, Nirma Skrbo3, 4, Siver A. Moestue1, Einar A. Rdland5, Eldrid Borgan1, 6, Alexandr Kristian3, Beathe Sitter1, 7, Tone Frost Bathen, 28, Anne Lise Brresen-Dale4, 6, Gunhild M. Maelandsmo3, 9, Olav Engebrten4, 10, Therese Srlie6, Elisabetta Marangoni11, Ingrid Susann Gribbestad, 28

1Dept. of Circulation and Medical Imaging, NTNU, Trondheim, Norway; 2St. Olavs University Hospital, Trondheim, Norway; 3Dept. of Tumor Biology, Institute of Cancer Research, Oslo University Hospital Radiumhospitalet, Oslo, Norway; 4Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway; 5Dept. of Informatics, University of Oslo, Oslo, Norway; 6Dept. of Genetics, Oslo University Hospital Radiumhospitalet, Oslo, Norway; 7Dept. of Technology, Sr-Trondelag University College, Trondheim, Norway; 8Dept. of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; 9Dept. of Pharmacy, Faculty of Health Sciences, University of Troms, Troms, Norway; 10Dept. of Oncology, Oslo University Hospital Radiumhospitalet, Oslo, Norway; 11Preclinical Investigation Unit, Translational Research Department, Institut Curie, Paris, France

We employed high-resolution magic angle spinning (HR MAS) MR spectroscopy and gene expression microarray to map the metabolomic and transcriptomic characteristics related to choline metabolism in large panel of patient-derived breast cancer xenografts (N=34) and to evaluate the clinical relevance of xenograft models for metabolomic studies. The results showed significantly different choline metabolic and gene expression profiles in luminal B and basal-like subtypes of breast cancer. It also indicated that the patient-derived xenografts are representative of human breast cancer, and may be valuable for further exploration of subtype-specific metabolic and transcriptomic traits.

Keywords

abnormal addition alpha alters analyses anticancer assays attention audience auger basal basic biology breast cancer cells characteristics characterized chemotherapy choline circulation classified clinical cohort college combined comparing component concordance consistent containing correlated correlation curie dale dept derived detected domain drug drugs earlier encoding epithelial evaluate exploration explore expression expressions faculty feature fully gene genes genetics glycine health highly hospital human identification in vivo indicate indicates informatics institute interplay investigated investigation involved lactate like likely loading magic malignant mammary maria mechanisms medical medicine membrane metabolic metabolism metabolites model models molecular negative oncology panel pathway patient patients pattern pharmacy play plot positive principle profiles properties proteins regulation relevance relevant renowned represent representative resistance resolution retrieved role samples sciences scientists score sensitivity several significantly sitter special specimens spectra spectrometer spectroscopic spin spinning strong studies subtype subtypes suggest target targeted targets technology tissue tone traits translational tumor tumors underlying understood unit unknown valuable variable vary vitro water