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Abstract #3899

Multi-Modal Imaging to Evaluate the Effects of Novel TLR Agonist Adjuvants in Vaccine-Mediated Tumor Immunity

Deepak K. Kadayakkara1, Hyam I. Levitsky1, Jeff W.M. Bulte2, 3

1Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD, United States; 2Cellular Imaging Section, Institute for Cell Engineering,, Johns Hopkins University, Baltimore, MD, United States; 3MRI research division, Johns Hopkins School of Medicine, Baltimore, MD, United States

We evaluated the effects of novel adjuvants in cancer vaccines using non-invasive imaging. Magnetovaccination and MRI were used to evaluate dendritic cell-mediated antigen capture and delivery to regional lymph nodes. The ensuing effector immune response mediated by T cells was studied by antigen-specific transgenic T cells and bioluminescence imaging. We show that, unexpectedly, adjuvants reduced antigen delivery to lymph nodes; however they induced a more effective tumor therapeutic response suggesting an alternate and hitherto unreported pathway for T cell expansion and accumulation.

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Keywords

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