Meeting Banner
Abstract #2096

Histological Correlation of DKI-White Matter Modeling Metrics in the Cuprizone-Induced Corpus Callosum Demyelination

Maria F. Falangola1, 2, David Guilfoyle3, Edward S. Hui1, Xingju Nie1, Ali Tabesh1, Jens H. Jensen1, Scott Gerum4, Caixia Hu4, John LaFrancois4, Heather Collins1, Joseph A. Helpern1, 2

1Radiology and Radiological Science, Center for Biomedical Imaging, Medical University of South Carolina, Charleston, SC, United States; 2Neurosciences, Medical University of South Carolina, Charleston , SC, United States; 3Nathan Kline Institute, Orangeburg,, NY, United States; 4Nathan Kline Institute, Orangeburg, NY, United States

The goal of this study was to utilize a recently developed white matter modeling method compatible with diffusional kurtosis imaging to characterize the demyelination process in the corpus callosum of the cuprizone mouse model. We studied 10 cuprizone and 10 control mice for a period of 10 weeks. All diffusion metrics were derived from DKI datasets. Quantitative analysis of myelin density was performed using Solochrome staining. The histological correspondence shown in our results demonstrate the correspondence between the WMM metrics and a well established histological marker of myelin.

Abstract PDF Poster PDF

Keywords

acknowledgments acquisition affected almost animal assess assessed assessment assumed audience axial axons best better biomedical biophysical brains caudal characterization closely complete considered consistent containing control controls copper corpus corrected correlation decreased decreases degree density derived diet diets differentiated differentiation differentiators differently diffusion diffusional diffusivity digital dimensionless disease distribution drawn eliminate entire estimation estimator except expected extra fibers five fraction fractional gradient greatest heather help heterogeneity histological histology in vivo include indicated induced inside institute integrity intense intensity intrinsic investigate kurtosis least maintained manually maps maria matrix measured medical metric metrics mice middle mirrored mirroring model modeling models morphological mouse occurs overall parametric partial particularly pictures processed proposed quantitative quantitatively radial radiological radiology reduce reduced reflecting related relating reports reproducible resolution respectively respiration response science shot sigma significantly similarly slice south space stained staining strongest suggest suppl supported system table target tensors throughout tissue treated units utility validate volume water weeks white yield yielded