Meeting Banner
Abstract #0337

A Novel Neuroprotective Strategy Using Methylene Blue---A Longitudinal MRI Study

Qiang Shen1, Fang Du1, Shiliang Huang1, Yash Vardhan Tiwari1, Timothy O. Duong2

1Research Imaging Institute, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States; 2Research Imaging Institute, UT Health Science Center at San Antonio, San Antonio, TX, United States

Methylene blue (MB) has unique energy-enhancing and antioxidant properties. It has been recently demonstrated that MB substantially reduces infarct size in transient focal cerebral ischemia in rats by histology. As the next logical step, in this work, we evaluated MBs neuroprotective effects using non-invasive magnetic resonance imaging (MRI) to longitudinally evaluate ischemic evolution. Comparisons were made with functional changes using neurological assessments. Our results showed that MB significantly reduces infarct size and behavioral deficit in the 60-min transient cerebral ischemia in rats. MB salvaged markedly more initial core and mismatch tissues than vehicle.

Keywords

according acts acute allows already animals antioxidant apparent applied arterial auto automated behavioral blood blue bypassing cerebral chain chem clinical clinically consistent consumption contrast control core correction cyanide cycler dead decline deficit defined deprivation derived diffusion disease double edema either electron electrons enable enhancer equal evaluate evaluated evolution falling fang fast final firmly flow focal fully functional future glucose grew health hemisphere histology immediately impairment improved incorporate indications indicative infarct infusion initial injury investigate lesion logical longitudinal made malaria male maps maximize measured metabolic methylene minimize mismatch models neurobiology neurological neurologically next novel occlusion oxidizing oxygen paired percentage pixel pixels plus post production progress properties property quantitative rats reactive recently recovery redirect reduce reduces registered routinely salvaged science significantly smaller species speedy spin spontaneous status step strategy stroke studies subjected substantially suture taken texts therapy thereby threshold tissue tissues transient translation transport treated twelve unique variance vehicle vitro volume volumes walking