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Abstract #4471

Alginate Encapsulated Lactate Dehydrogenase and Hyperpolarized MR as a Platform to Characterize Enzyme Kinetics

MAGNA25Deborah K. Hill1, Kayvan R. Keshari2, Renuka Sriram2, Bertram Koelsch2, Mark Van Criekinge2, Yuen-Li Chung1, Thomas R. Eykyn1, 3, Martin O. Leach4, John Kurh

1Clinical Magnetic Resonance, CRUK & EPSRC Cancer Imaging Centre, Royal Marsden NHS Trust & The Institute of Cancer Research, Sutton, Surrey, United Kingdom; 2Radiology and Biomedical Imaging, University of California, San Francisco (UCSF), United States; 3The Rayne Institute, Lambeth Wing, St. Thomas Hospital, London, United Kingdom; 4Clinical Magnetic Resonance, CRUK & EPSRC Cancer Imaging Centre, Royal Marsden NHS Trust & The Institute of Cancer Research, Sutton, Surrey, United Kingdom

Hyperpolarized MR provides a means to acquire real time in vivo metabolic rates, such as the kinetics of lactate dehydrogenase, through the dramatically increased SNR of 13C substrates. The modeling of these kinetics is complicated by the number of parameters which can influence the derived rate. Ex- vivo studies that modulate these parameters are useful in delineating their impact. We present the use of alginate microspheres to encapsulate LDH for use in a bioreactor, thus providing a controlled cell-like environment, to help elucidate the complicated kinetic system governing hyperpolarized 13C kinetics.

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