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Abstract #4469

In Vivo Analysis of Pseudomonas Auriginosa Bacteria and the Effects of a Novel Bacterial Molecule by Whole Cell High Resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy

Valeria Righi1, 2, Meenu Kesarwani3, Laurence Rahme3, Aria A. Tzika, 24

1NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burn Institute, Harvard Medical School,, Boston, MA, United States; 2Athinoula A. Martino Center Biomedical Imaging Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States; 3Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burn Hospital, Harvard Medical School, Boston, MA, United States; 4NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burn Institute, Harvard Medical School, Boston, MA, United States

High Resolution Magic Angle Spinning (HRMAS) NMR spectroscopy was applied to determine the metabolite profile of live Pseudomonas aeruginosa bacteria. One-dimensional and 2-dimensional HRMAS NMR was performed on intact bacterial cells represents a promising method that could provide in vivo information of metabolomics in live bacteria. This in vivo NMR biomedical technique may prove to be a helpful tool in gene function validation, the study of pathogenesis mechanisms, the classification of microbial strains into functional/clinical groups and the testing of anti-bacterial agents and to distinguish the metabolic profile of different mutants.

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Keywords

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