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Abstract #4363

Stearic-Polyethylenimine Modified SPIO Nanoparticles for MRI of Gene Delivery to Liver

lin Gao1, zhiyong Wang1, lisi Xie2, xiaojing Long1, zhiying Chen3, bensheng Qiu1, 4

1Paul C Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China; 2Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Gaungzhou, Guangdong, China; 3Gene and Cell Engineering Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China; 4Department of Radiology, University of Washington School of Medicine, Seattle, WA, United States

An amphiphilic polymer, stearic-polyethylenimine (PEI600) was synthesized and used for modified hydrophobic supermagnetic iron oxide (SPIO) nanocrystals via self-assemble progress in aqueous phase. The nanocomposite system is novel magnetic imaging contrast agents and capable to bind and deliver plasmid DNA for gene transfection. in vivo, it was observed that MR signal decreased significantly via a clinical 3.0T MRI scanner. The histochemistry of tissue sections of liver results showed that this nanoparticle gene delivery system provided a safe and efficient method for gene delivery with non-invasive imaging monitoring capability.

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Keywords

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