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Abstract #4360

Improved Identification of Ferritin-Tagged Grafts in Mouse Heart at Higher Magnetic Field Strength

Anna V. Naumova1, 2, Vasily L. Yarnykh1, 2, Niranjan Balu1, 2, Hans Reinecke, 23, Charles E. Murry, 23, Chun Yuan1, 2

1Radiology, University of Washington, Seattle, WA, United States; 2Center for Cardiovascular Biology, University of Washington, Seattle, WA, United States; 3Pathology, University of Washington, Seattle, WA, United States

Mouse skeletal myoblasts were genetically modified to overexpress ferritin, a natural iron storage protein. T2 relaxivity of ferritin-tagged cells dramatically increased with increase of the magnetic field strength; thus improves MRI graft identification and assessment of the graft size.

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Keywords

acquisition advantages aims alter animal applications approaches assessed assessment better biology cardiac cardiovascular cell cells chem cine clinical compensated contrast damaged decreased described detection differentiation dilution dividing echoes ensuring equally evaluation fate field flow gene genetically gradient graft grafts heart host identification important improve improved in vivo included indistinguishable induced infarctions injured intensity invasive iron label labels live long longitudinal matrix measured middle month mouse myocardial myocardium natural nature organ particles pathology pellets plasmid potential potentially previous proliferation properties protein protocol quantify radiology reagent repair reporter resolution scanned scanner scanners shorter significantly skeletal slice spaced spectrometer spin stably statistically stem storage strength strengths studies tagged therapy tissue tissues tracking transduction transplantation transplanted type unlabeled vector ventricle viability view visualized vitro wild yuan