Meeting Banner
Abstract #4090

Chemical-Shift MRI Measurements of Variations in Murine Brown Adipose Tissue Fat Content Due to Housing Temperature

Houchun Harry Hu1, 2, Daniel Larry Smith, Jr. 3, Yongbin Yang3, Guihua Zhai4, Tim R. Nagy3

1Radiology, Children's Hospital of Los Angeles, Los Angeles, CA, United States; 2Electrical Engineering, University of Southern California, Los Angeles, CA, United States; 3Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL, United States; 4Radiology, University of Alabama at Birmingham, Birmingham, AL, United States

Brown adipose tissue (BAT) is involved in non-shivering thermogenesis. When stimulated, it metabolizes its fat stores to produce heat and maintain body temperature. We utilize chemical-shift MRI at 9.4T to measure BAT fat content in 3 groups of mice (n=16/group) that were singly housed at 16-cold, 23-room, and 30-warm degrees Celsius over four weeks. We demonstrate that BAT fat-signal fraction measured at week 4 is positively correlated with housing temperature (cold-50.48.0%, room-60.811.9%, hot-79.412.1%) and significantly different between groups (p<0.01). Results demonstrate the differential thermal demand of animals in each group and prove that chemical-shift MRI can reflect BAT activity in vivo.

Abstract PDF E-Poster Video

Keywords

ability access activity adipose ambient animal animals arrows association attributed axial balance body brown chambers channel characterization characterize chemical chow coil coincident cold composition consumed content conversely cover cycles decrease decreased degrees demand demonstrating depot depots described despite determined diet differential dorsal duration eight either electrical energy engineering environmental evident exhibited experiment extraction feasibility food forty fraction frequency furthermore gain gained gains gating global greatest highest histology hospital hour housed housing hypothesize ideal identify illustrates in vivo intake involve leads lean lowest male maps mass matrix measure measured measures medical metabolism metric mice minimal minutes models nearly noticeable nutrition obesity optimal plots positive powers predominantly primarily progressively protein protons quantitative radiology reduction reeder reflect refs relationship reported representative respiratory risk rodent role room scientific shivering significantly singly slices smallest smith strong studies summarize surface system systematically systems temperature thermal throughout tissue topic translates utility utilize utilized variations warm water week weekly weeks yang