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Abstract #4069

Imaging the PH Changes in a Glycerol-Induced Acute Kidney Injury Mouse Model with a MRI-CEST PH-Responsive Contrast Agent

Dario Livio Longo1, Alice Busato1, Stefania Lanzardo2, Federica Antico3, Silvio Aime1

1Molecular Imaging Center, University of Torino, Torino, Italy; 2Department of Clinical and Biological Sciences, University of Torino, Torino, Italy; 3Department of Internal Medicine, University of Torino, Torino, Italy

pH is an important microenvironment parameter, in fact a number of disease states are associated with alterations in pH, such as ischemia, inflammation, infection and cancer, and many kidney injuries are associated with a perturbation of renal pH, therefore imaging techniques able to report in vivo on pH modifications would have a great relevance. The aim of the work was to investigate the use of Iopamidol as a MRI-CEST pH reporter of the disease evolution in glycerol-induced AKI model, exploiting the capability to image pH values in different regions for single kidneys.

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Keywords

able acidosis acute agent allows altered amount animals approaches approved assess assessed biological biopsy blood body calculation casts catheter chemical clinical closely complete considerable contrast control correlated damage days decrease denote diagnostic dilatation disease dosages dose doses evaluate evaluating evolution exchange fast fixed function functionality glycerol healthy hind histology in vivo induced induction inferior injected injection injury internal interpolated intramuscular invasive investigated investigations iodine kidney lesion light like limbs looking loss medicine mice microscopy model molecular monitor mouse nature newer nitrogen occurred onset paraffin pathological peak perturbation physiology pixels preceded primary proposed pulse recently recover recovery reduced relevance reliable renal respectively responsive restoration reverting river sacrificed samples saturation scanner sciences sectioned serum slight smoothing solution spectra spin splines stage start starting surrogate tail third transfer tubular urea variations vein week weeks