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Abstract #4055

Assessment of AKI Severity in an Ischemia/reperfusion Mouse Model Using T2 Mapping: Comparison with Neutrophil Gelatinase Associated Lipocalin (NGAL) and Kidney Injury Molecule-1 (KIM1)

Jan Hentschel1, Lajos Marko2, Erdmann Seeliger3, Dominik N. Mller2, 4, Andreas Pohlmann1, Thoralf Niendorf1, 2

1Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrck-Center for Molecular Medicine (MDC), Berlin, Germany; 2Experimental and Clinical Research Center (ECRC), a joint cooperation between the Charit Medical Faculty and the Max-Delbrck-Center, Berlin, Germany; 3Institute for Vegetative Physiology, Charit Medical Faculty, Berlin, Germany; 4Department of Experimental Medicine I, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University, Erlangen-Nrnberg, Germany

Renal medullary hypoperfusion and hypoxia play a pivotal role in acute kidney injury (AKI). This study examines the correlation between MRI and NGAL/KIM1 in mouse AKI including ischemia/reperfusion injuries of different severity to elucidate the link between changes in T2 and kidney physiology. The T2 values derived from the medulla and cortex of clipped kidneys showed a strong correlation with NGAL. A similarly strong correlation was observed between KIM1 and T2 deduced from the medulla. This indicates that T2 -mapping may be useful as a non-invasive marker for the characterization of pathophysiological changes in mouse models of AKI.

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Keywords

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