Meeting Banner
Abstract #3052

Dynamic Metabolic Signatures of Metastatic and Non-Metastatic Breast Cancer Cells

Rui Vasco Simoes1, Ellen Ackerstaff1, Natalia Kruchevsky1, Yang Pu, 12, George Sukenick1, Jason A. Koutcher1

1Memorial Sloan-Kettering Cancer Center, New York, NY, United States; 2The City College of New York, New York, NY, United States

Distinguishing non-invasively metastatic from non-metastatic clinical breast cancer, would significantly improve evaluation of patients prognoses. Two isogenic murine breast cancer lines, metastatic 4T1 and non-metastatic 67NR, were studied in an NMR-compatible cell perfusion system to investigate their dynamic metabolic responses to (a) oxygen availability vs. hypoxia, and (b) glutamine supply vs. deprivation. When exposed to these stress conditions, each cell line adopted different metabolic strategies. Non-metastatic 67NR cells revealed overall higher glycolytic activity than metastatic 4T1 cells. In the more aggressive cell line, glycolysis is glutamine-dependent during hypoxia, and decreases when oxygen becomes available to favor TCA cycle activity.

Abstract PDF E-Poster Video

Keywords

accumulation activity adopts aired alanine alone analyses ares assay availability back bore breast cancer carried cell cells changing chemical city college comp compounds concentration contains cope counted culture curve cycle days decreased dependent depending deprivation derived determine developing dies distinguishing dropping drops duplicate dynamic ells exposed faster favor fold generates glucose glutamate grant grants green grow growth guava hypoxia in vivo incubated initial initially insight intro invasive kept labeled lactate latter lipids loaded made magnet major mate media medium meta metabolic metabolite metastatic mixed mobile modified normalized nutrient ongoing oxygen peak pendent perfused perfusion perturbations plastic plus position presence privation production prognosis recent refer remains responses revealed said samples screw scribed seeded signature significance significantly spectra stage stages statistical steps still stress stud studied studies supplemented supply support synthesis system technologies tensions therapies tube tumor unique unlike useful vertical vitro water yang