Meeting Banner
Abstract #3012

Maximizing Dendritic Cell Migration After Vaccination Therapy

Mangala Srinivas1, Fernando Bonetto2, Erik Aarntzen1, Cornelius JA Punt3, Otto C. Boerman4, Wim J. Oyen4, Pauline Verdijk5, Carl Figdor1, Arend Heerschap6,

1Tumor Immunology, RUNMC, Nijmegen, Gelderland, Netherlands; 2INTEC-CONICET, Argentina; 3Oncology, RUNMC, Netherlands; 4Nuclear Medicine, RUNMC, Netherlands; 5Vaccinology, RIVM, Netherlands; 6Radiology, RUNMC, Netherlands

Dendritic cell (DC) vaccination is an emerging therapy for cancer patients. Injected DCs must migrate to lymph nodes for immunostimulation. However, migration is extremely poor (<4% upon intradermal injection). Expense, logistic and sensitivity issues hinder clinical optimization. Therefore, we developed a quantitative, In Vitro 19F MR-based migration assay using 3D collagen scaffolds and tissue samples, and validate our results using clinical data acquired using scintigraphy on 111In-labeled DCs. We found a strong relationship between migration rates and total cell numbers. We also studied the effect of different components such as cytokines on migration both In Vitro and in the clinic.

Abstract PDF

Keywords

able activate added aging allows alternatively although always antigen assay assays assessments bars biological blood blue bolus cancer carried cell cells cellular challenging chap cheap chem cine clinical coil component control correlation costs cure customizable develop developed difficult ding directly done draining dropped drug efficient eight embedded emulsion ever example expensive finally find fluorocarbon furthermore gradient grants greatly handle health horizontal hours human immune immunology in vivo indicated inherently injections interact intra involving issues known labeled labeling layer lymphocytes manner matrix maximizing medic melanoma microscopy migration migratory million millions mimics modified monitoring movement necessary nodes nonspecific nuclear often opaque optimization optimize paper part patient patients percentage peripheral placed predict principally proved punt quantitative quantitatively reached reaching reason recent recently reduced respectively sample samples scaffold sensitive spin stimulate subsequently suffered suggest suitable system target temperature tens therapies therapy tissue together towards tracking transferred trend trials typically umbers upward vaccination validate vertically vista vitro whether