Meeting Banner
Abstract #1773

Investigation on Neuroprotective Role of Caffeine Against MPTP Induced Neurotoxicity in Mice Using 13C NMR Spectroscopy

Puneet Bagga1, Suresh Kumar M1, Anant Bahadur Patel1

1NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India

MPTP is a well established neurotoxin which causes selective degeneration of dopaminergic neurons in substantia nigra leading to dopamine loss and hence PD. Neuroprotective effects of caffeine, a MAO-B inhibitor against MPTP were investigated using infusion of [1,6-13C2]glucose and 1H-[13C]-NMR spectroscopy in adult male C57BL6 mice. Treatment with MPTP led to loss of glutamatergic and GABAergic activity in cortex, striatum, thalamus-hypothalamus and olfactory bulb. Pretreatment with caffeine led to recovery of glutamatergic and GABAergic activity in cortex and olfactory bulb, but the same in striatum and thalamus-hypothalamus was only partially recovered, suggesting partial neuroprotection conferred by caffeine against MPTP neurotoxicity.

Abstract PDF

Keywords

acids administration affecting amino anesthetized animal approved biology blood brain bulb caffeine cellular cerebral chronic committee concentration concluded control correlated cortex cycle days debilitating decreased disease disorder enrichment entire ethics evaluate extracted extracts fasted flow frozen function funding glucose hence human humans hypothalamus impairment indicating induced infused infusion institute intravenously investigation involved labeling liquid male materials mechanisms metabolic metabolites mice model models molecular month mouse negatively neural objective occurrence olfactory overnight oxidation partial partially people population pretreatment primate prior protection protocols received recovery regional revealed rodent role saline science significantly situ spectra spectroscopy studied studies supported technology thalamus tissue together treated treatment twice uptake urethane years