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Abstract #1542

NMR Metabolomic and LIBS Elemental Profiling of Anticancer Herbal Formulation

Rama Jayasundar1, Gaurav Sharma1, Shyam S. Chauhan2, Thirumurty Velpandian3

1Department of NMR and MRI, All India Institute of Medical Sciences, New Delhi, India; 2Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India; 3Department of Occular Pharmacology, All India Institute of Medical Sciences, New Delhi, Delhi, India

The study evaluated the metabolic profiling, elemental analysis and anticancer potential on four polyherbal formulations namely KG, VK, MK and GTK using NMR, LIBS and MTT assay, respectively. Antioxident potential of formulations was carried out using FRAP assay. Amongst four formulations maximum cytotoxicity was observed for KG (IC50 22.48 lower case Greek mug/ml)followed by MK (IC50 27.58 lower case Greek mug/ml). Using FRAP assay maximum antioxidant potential was achieved for VK (17.0 lower case Greek muM Fe++g-1) amongst four. KG and MK formulations were selected for characterization through NMR Spectroscopy, which revealed the chemical indentification of metabolites to explore pharmacological action. LIBS data supports antioxident properties of formulations.

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Keywords

acid acids acquisition action activity amino among anticancer antioxidant aqueous aromatic assay assessed assigned assignments biochemistry break bromide butyrate cancer capacity carbon carried cell cells centrifuged channel characterization chemical choline coaxial composition compounds concentration contain counts critical cytotoxic definite delay diluted dimensional distilled elemental employed energy establishing evaluated expanding explore extracted extracts ferric filtered fingerprinting five flow form formulation formulations glycerol good gram herbal hydroxy identification important induced ingredients inhibit inhibitory insert insoluble institute integrated intensity japan laser library libs liver made make materials medical medicinal medicinally medicine medicines metabolic metabolites minutes nuclei ocean optics organic paper pathways peaks pharmacological pharmacology piper plant plants polar potential power profiling proliferation properties proton quality rapidly reducing repetitive resolving respect respectively revealed sample sciences selected settle several significantly solution spectra spectral spectrometer spectroscopy spectrum studied studies sugars sulphate supports system tablet taken taking tube untreated viability water wavelength