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Abstract #0960

Continuous Infusion of Manganese Improves Contrast & Reduces Side Effects in MEMRI Studies

Dana S. Poole1, Nathalie Doorenweerd1, Ahmed Mahfouz1, 2, Marcel J.T. Reinders2, Louise van der Weerd1, 3

1Radiology, Leiden University Medical Centre, Leiden, Zuid Holland, Netherlands; 2Computer Vision Lab, Delft University of Technology, Delft, Netherlands; 3Anatomy and Embriology, Leiden University Medical Centre

Few studies until now have used manganese-enhance MRI (MEMRI) for general phenotyping of transgenic animals. A high dose of manganese ensures a high quality and contrast of the acquired images while reducing the acquisition time, which is beneficial for phenotyping studies containing large numbers of animals. In the present study, we deliver successfully to C57Bl6J mice the highest dose attempted so far: 480mg/kg MnCl2, achieving a high contrast while circumventing weight loss or visible signs of distress. This manganese administration protocol will be of particular use to study brain activation patterns occurring over several days, such as in transgenic or chronic disease models.

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