Meeting Banner
Abstract #0918

Pre-Symptomatic Cerebellar Lesions & Ventricle Enlargement in an EAE Mouse Model Revealed by Microscopic MRI

Stefano Lepore1, 2, Helmar Waiczies1, 2, Jan Hentschel2, Jason M. Millward3, 4, Carmen Infante-Duarte3, 4, Thoralf Niendorf, 24, Sonia Waiczies1, 2

1Ultrahigh Field Imaging in Neuroinflammation, Experimental and Clinical Research Center (ECRC), a cooperation of the Charit Medical Faculty and the Max Delbrck Center for Molecular Medicine, Berlin, Germany; 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany; 3Experimental Neuroimmunology, Charit Universittsmedizin Berlin, Berlin, Germany; 4Experimental and Clinical Research Center (ECRC), Charit Universittsmedizin Berlin, Berlin, Germany

Microscopic MRI enables the acquisition of highly resolved images in a reasonable scan time, thereby providing the possibility to perform in vivo longitudinal studies in animal models. In this study we have detected and tracked early structural changes in brain tissue of EAE mice prior to neurological symptoms. We were able also to detect a significant enlargement of the ventricles before disease onset and an increase in T2 relaxation time of CSF after disease onset. Microscopic MRI and T2 maps provide the opportunity to better understand the early processes involved in the development of encephalomyelitis.

Abstract PDF Poster PDF

Keywords

able acquisition aims alteration alterations animal animals appearance auto brain central cerebellar cerebellum choroid clinical coil comparing cooled coronal correlated correlation daily days defined described detect determine development disease diseases distribution early ease echoes enables enlargement experimental explored female field fluid furthermore head highly homeostasis hypo identified immunization in vivo induced induction inflammation involved larger lateral lesions longitudinal loss manifestation maps medical medicine mental mice micro microscopic model models modifications molecular monitor myelitis neurological noticed onset period plexus possibility post preventing previously prior processes producing providing qualities reasonable require resolution revealed scenario sclerosis score scoring slice slices slowing spatial starting structural studies symptom symptoms system tech tissue tracked understand useful ventricle ventricles volume water weeks weighed