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Abstract #0895

Metabolite Quantitation in Experimental Demyelination Model Using In-Vitro MRS: A Pilot Study

Naranamangalam Raghunathan Jagannathan1, Krithika Balasubramanian1, Uma Sharma1, Senthil S. Kumaran1

1Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, Delhi, India

The objective was to characterize and quantitate metabolites in demyelinated rat brain tissues and compare it with the normal tissues of control rats by in-vitro 1H-MRS. Overall 28 metabolites were unambiguously assigned. The concentration of N-acetyl aspartate (NAA) reduced while that of alanine (Ala) and taurine (Tau) increased in demyelinated tissues. Decreased NAA is attributed to breakdown of myelin and increased Tau is related to decreased signal transmission across the demyelinated neurons. Ala serves as a nitrogen carrier between glutamatergic neurons and surrounding astrocytes and its increase may be attributed to damage to these neurons leading to its accumulation.

Keywords

absolute achieved acid acute addition advantage amino amount appeared assigned assignments attributed beginning best better biochemical biochemistry biological blood body brain breakdown carried cells cerebral characterization choline comparing comprehensive concentration concentrations conditions considered control controls correlated correlation damage decrease delay density detailed detection determined development dimensional disease donor early elevated enables epilepsy evaluation even experimental extract extraction formate frozen functions gain glucose glutamate hence humans hypoglycemia hypoxia identified implicated indicate induced inflammatory inhibitory initiation institute integrated isolated leading lesion localized male many marker medical membrane metabolic metabolism metabolite metabolites model models molecular monitor multiplets neuronal neurons onset pathological pathology potential presence pressure primarily probability proton protons rats reduced regulation regulator related reported respectively revealed sacrificed sample sclerosis serve served severity sheath since snap spectra spectral spectrometer spectroscopy spectrum spine stabilization stage started statistical studies subjected suggested suggests swell synthesis table tissue tissues tool transmission understand understanding various vitro water wherein width