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Abstract #0894

Intracellular Oximetry Using in vivo Fluorine-19 MRI as Biomarker of Immunotherapeutic Response of Cytotoxic T Cells in Mouse Glioma

MAGNA25Jia Zhong1, 2, Masashi Sakaki3, Hideho Okada3, 4, Eric T. Ahrens1, 2

1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, United States; 2The Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA, United States; 3Brain Tumor Program, Cancer Institute, University of Pittsburgh, Pittsburgh, PA, United States; 4Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, United States

Immunotherapy using live cells is opening up new avenues for brain tumor treatment with minimal damage to healthy tissue. Noninvasive biomarkers of therapeutic efficacy is of great importance for the evaluation of emerging immunotherapies. In this study, we report the use of intracellular perfluorocarbon (PFC) labeling of glioma cells, combined with 19F T1 measurements, to assay glioma oximetry. We show that glioma oxygen level responds sensitively to the influx of therapeutic cytotoxic CD8+ T cells into mouse glioma. We also show that intracellular oximetry can detect the presence of sparse CD8+ T cell numbers, even in the absence of significant tumor shrinkage.

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Keywords

action additional among anesthetized animals antigen aspect atoms avenues biological biomedical brain calibration cancer caused cell cells chemotherapy circulation combined common control correlate curve cytotoxic damage days decreased delivery determined determining developed diagnosed diluted efficacy elevated elevation emerging emulsion encompassing entire ether evaluation exhibited feedback flow fluorine great harbor health healthy held hypothesis immune implantation implanted importance improvement in vivo incubation indicate infiltrating infiltration infused infusion injected injection inoculated inoculation inside institute intracellular invasive killing labeled labeling labels laboratories linear located location longitudinal longitudinally malignant mass matrix measure measured medium mice minimal model monitor mouse nonspecific oxygen oxygenation partial perfused physiology position post pressure problem progression promise pronouncedly quantitative radiation rare received receptor recurrence reduction relationship remained reported representative response revealed sacrificed saturation school sensitive sensitively shortening slice slices solid spike spin square staining step studies system tensions therapeutic therapies therapy thereafter tissue towards treated tumor unlabeled valuable versus visible vitro weeks year