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Abstract #0495

MR Detectable Metabolic Changes Associated with Mutant IDH

Sarah Woods1, Myriam Marianne Chaumeil1, Alessia Lodi1, Robert M. Danforth1, Aaron Robinson2, Joanna J. Philips2, Sabrina M. Ronen1

1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States

Our goal was to assess the MRS-detectable metabolic changes associated with mutation of the isocitrate dehydrogenase (IDH) gene in two isogenic glioblastoma cell lines: U87IDHmutant and U87IDHwild-type. 1H MRS of extracts confirmed the presence of 2HG and detected several metabolic changes including elevated phosphocholine and elevated lactate in U87IDHmutant compared to U87IDHwt. The pyruvate-to-lactate conversion as measured by hyperpolarized 13C MRS was significantly lower in U87IDHmutant cells likely due to a drop in lactate dehydrogenase activity. Our studies inform on the metabolic consequence of the IDH mutation and identify biomarkers of the mutation.

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Keywords

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