Meeting Banner
Abstract #0342

Renal Susceptibility Imaging in AT1 Deficient Mice

Luke Xie1, 2, Wei Li3, Matthew Sparks4, Yi Qi2, Gary P. Cofer2, Thomas Coffman4, G. A. Johnson, 12, Chunlei Liu, 23

1Biomedical Engineering, Duke University, Durham, NC, United States; 2Center for in vivo Microscopy, Duke University Medical Center, Durham, NC, United States; 3Radiology, Duke University Medical Center, Durham, NC, United States; 4Nephrology, Duke University Medical Center, Durham, NC, United States

This study utilizes the first renal susceptibility imaging on altered kidneys in mice lacking angiotensin II receptor AT1A or both AT1A and AT1B receptors. Kidneys were imaged using a GRE sequence at 9.4 T and susceptibility values were measured in cortex, outer medulla, and inner medulla for comparison in wild type (WT), AT1a -/- (A), and AT1a -/- AT1b -/- (AB) mice. AB kidneys exhibited interstitial fibrosis, tubular atrophy, and arterial thickening, which changed the spatial distribution of paramagnetic contrast for magnetic susceptibility. AB kidneys exhibited low contrast regions/vessels, higher susceptibility, and broader distribution compared to WT or A kidneys.

Abstract PDF Presentation Video

Keywords

acrylic allowed altered among anesthetized animals application arterial atrophy background biological biomedical brain broadly characterized chemical circle coarse collecting combined composition contrast cortex cortical decreased defect deficient defined depends determined deviation diffuse dispersed dissolved distinct distributed distribution distributions diverse double duke engineering explain explored extracted fibrosis field fields filter fixation focal formalin funded furthermore generally genetically gradient hand highly holder homozygous hours in vivo included inflammation inner interstitial invaluable kidney kidneys lack lead local long longer macromolecules magnet magnitude many material materials medians medical medulla mice microscopy mouse much nonetheless numerous oblong organ outer overall oxford paramagnetic particularly perfused perfusion plots preparation pressure produced promising quality quantification quantified quantify quantitative radiology receptor receptors reduced renal resolution respectively respond response sample shapes shrunken sparks spatial sphere squares stability stains stimulus stored structured structures superconducting support susceptibilities susceptibility system systemic table taken target thickening tool tubular tubules type types unwrapping validated variation vessels walls wild