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Abstract #4066

A Metabolomics Approach to Biomarker Discovery for Non-Alcoholic Steatohepatitis

Brittany Ann Lee1, Keisaku Sato2, Celine Baligand1, Huadong Zeng3, Parvesh Sharma1, Krista Vandenborne4, Arthur Edison5, Angela Dolganiuc2, Glenn A. Walter1

1Department of Physiology, University of Florida, Gainesville, FL, United States; 2Department of Internal Medicine, University of Florida, Gainesville, FL, United States; 3AMRIS, University of Florida, Gainesville, FL, United States; 4Department of Physical Therapy, University of Florida, Gainesville, FL, United States; 5Department of Biochemistry, University of Florida, Gainesville, FL, United States

Non-Alcoholic Steatohepatitis (NASH) is a lipid infiltration in the liver causing inflammation and damage that if left undiagnosed can lead to cirrhosis. Currently diagnosis occurs through biopsy only. Metabolomics is an approach of identifying and quantifying metabolites produced by an organism. In vivo magnetic resonance spectroscopy (MRS) and ex vivo higher resolution magic angle spinning (HR-MAS) MRS are analytical methods used in metabolomics. The goal of this study was to implement global metabolomics to find a non-invasive biomarker from differences in the NASH mouse model from the control, using in vivo and ex vivo MRS

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