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Abstract #4043

13C-NMR Spectroscopy in the Detection of 2-Hydroxyglutarate as a Novel Biomarker of IDH Mutation Status in Glioma

Hyeon-Man Baek1, Yun-Ju Lee1, Eun-Hee Kim1, Chaejoon Cheong1, Seung-Ho Yang2

1Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chungbuk, Korea; 2Neurosurgery St. Vincent's Hospital, Catholic University, Suwon, Gyeonggi-do, Korea

We present here that the data analysis of 1H- and 13C-NMR spectra of the tumor extracts can demonstrate a significant increase in the concentration of 2HG in IDH mutated tumors. On 13C-NMR spectra, 2HG peaks for each of the 5 carbons were detected in the IDH1 mutated but not IDH wild type tumors. 2HG 13C-resonances (C3, C4, C2, C1, and C5) were detected at 31.70, 34.18, 72.79, 181.95, and 183.56 ppm. It is expected that 2HG may be actively being produced during the period of 13C-substrate infusion (e.g., [U-13C]-glucose). Therefore, the present study demonstrates the feasibility of 1H- and 13C-NMR spectroscopy in the detection of 2HG as a novel biomarker of IDH mutation status in glioma.

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Keywords

abundance acid acquisition actively activities analyzed basic carbons catholic cellular chemical clinical complex concentration concentrations coupled dang decoupled decoupling detected detection deuterium dimensional disease division elevated elucidated evaluate examined expected external extracts feasibility five gene give glucose grade grades hospital human identified in vivo indicates infusion institute intensities isotope labeled locations measured metabolic metabolism metabolite metabolites methylene molecule multiplets mutant mutated mutation mutations natural nature neurosurgery novel oxide part particular pathways patients peaks period phenotype plus positions possibility probe produced profiling prognosis pulse quantification recently redissolved resolution sample samples scanned science secondary separated serve singlets solution somatic spectra spectral spectrometer spectroscopy spectrum stable status stratification structure studies supported sweep system tissues transients tumor tumors type vitro waltz width wild yang