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Abstract #4020

Studies on Metabolic Alterations Due to Chronic Cold Stress: An NMR Based Metabonomics Apporach

Sonia Gandhi1, Subash Khushu1, Shubhra Chaturvedi2, Rajendra P. Tripathi3

1NMR Research Centre, Institute of Nuclear Medicine & Allied Sciences, Delhi, India; 2Division and Cyclotron & Radiopharmaceutical Sciences, INMAS, Delhi, India; 3NMR Research Centre, INMAS, Delhi, India

Chronic cold stress exposure results in increased sympathetic activity, immune suppression, gastrointestinal mucosal damage, affects central nervous system (CNS), hypovolemia, negative electrolyte balance & potential dysfunction to organs. Our study investigates the changes in metabolic profiles of urine in rats due to chronic cold stress & recovery using NMR spectroscopy. Results showed an increase & then decrease in metabolites such as 2-oxoglutrate, citrate, fumarate & N-methylnicotinamide indicating inhibition of TCA cycle & acclimatization to cold exposure. Significant increase in branched amino acid indicates kidney dysfunction. These studies can detect early biomarkers for cold stress injuries & develop strategies to combat variable climatic conditions.

Keywords

accepted acclimatization acclimatized achieved acid acquisition activities activity affects aims alanine allied alterations altered analytical anxiety arises behaviors biochemical biological body branched butyrate cages cardiovascular cause cells central centrifuged chronic citrate cold collected comparable component concentration concentrations conditions continuous control controls cope cycle cyclotron days decrease decreases delay demand detect developing directly disturbed duration dysfunction electrolyte electron emergence endocrine energy exacerbation exposed exposure flora formate gastrointestinal glucose help hence hydroxy hypoxia identifying important indicates indicating induced inhibition intake kidney lock lumen male mass material mechanism medicine mesh metabolic metabolism metabolite metabolites methyl microbes mixing modifications molecular monitoring negative normalization nuclear objectives particle pathways period post potential powerful prepared process product production products prolonged psychiatric rats recovery reflected regulated related remained respectively risk room sample samples sciences screening serves severe slight spectra spectral spectroscopy steel strategies stress studied suggests suppression sympathetic system table temperature thereafter transient transients transport unchanged urine various water