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Abstract #4002

In Vivo 1H MRS Metabolic Profiles in Gad1 Haploinsufficient Mouse Brain

Su Xu1, 2, Elizabeth M. Powell3, Andrew D. Marshall1, 2, Rolicia F. Martin3, Rao P. Gullapalli1, 2

1Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States; 2Core for Translational Research in Imaging @ Maryland, Baltimore, MD, United States; 3Department of Anatomy & Neurobiolog, University of Maryland School of Medicine, Baltimore, MD, United States

The B6-Gad1tm1.1Bgc mouse expresses decreased levels of glutamic acid decarboxylase (Gad67), the main enzyme that converts glutamate to GABA and presumably are found in epilepsy, schizophrenia and bipolar disorders. Although effective therapies seek to increase GABA levels in vivo, GABA levels have never been established in vivo, nor has the potential compensatory regulation of excitatory neurotransmitters, such as glutamate in this animal model. In the present study, we demonstrate the feasibility of using in vivo high resolution localized 1H MRS in studies of B6-Gad1tm1.1Bgc mouse brain at 7 Tesla to show regional differences in neurotransmitter levels.

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Keywords

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