Meeting Banner
Abstract #3891

Visualization of Therapeutic Angiogenesis by a Polymer-Based Magnetic Resonance Contrast Agent

Jun-ichiro Jo1, 2, Ichio Aoki1, Tsuneo Saga1, Yasuhiko Tabata3

1Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan; 2Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University , Kyoto, Japan; 3Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

The objective of this study is to design and prepare a new polymer-based contrast agent of magnetic resonance imaging (MRI) for the evaluation of therapeutic angiogenesis. Diethylenetriaminepentaacetic acid (DTPA), cyclic peptide containing an arginine-glycine-aspartic acid sequence (cRGD), and gadolinium ion (Gd3+) were introduced to dextran to obtain the dextran-based contrast agent (cRGD-dextran-DTPA-Gd). The cRGD-dextran-DTPA-Gd showed a higher longitudinal relaxivity compared with DTPA-Gd. The ischemic-angiogenic region could be clearly detected with MRI by the intravenous injection of cRGD-dextran-DTPA-Gd to mice with hindlimb ischemia. It is concluded that the cRGD-dextran-DTPA-Gd is a promising material to evaluate the therapeutic angiogenesis.

Abstract PDF Presentation Video

Keywords

accompanied accurately acid acquisitions activated affinity agent agents among animal application arginine arrows arteries assistance authors calculation cells chemical chemically circumflex circumstances clearly clinical concentration concentrations containing contrast created cyclic days deep deliver delivery designed detected develop dimensional effective enabled endothelial engineering essential evaluate evaluation expected experimentally expressed external femoral field formula frontier furthermore gadolinium glycine healed healing histological iliac indicate induction inherent injection institute intensity intravenous introduced invasive ions japan labeled laser ligated ligation like localized longitudinal matrix medical mice modalities modality model models modified molecular monitoring mouse national naturally normalized open optimal peptides picture placed polymer position positive prepare process profiles progress pulse radiological recent recovery regenerated regeneration repaired repetition representative required residue resolution respectively revealed saga saturation sciences selectively side slice solid spatial spin staining strongly structure successfully supine suppression surgically taken technical technology thank therapeutic therapy tissue tissues together treatments trials various vascular veins view visualization