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Abstract #3313

Functional Connectivity of the Mouse Brain Is Influenced by State of Consciousness: A Comparison of Awake and Differentially Anesthetized Mouse rsfMRI Protocols.

Elisabeth Jonckers1, Rafael Delgado y Palacios1, Disha Shah1, Caroline Guglielmetti1, Marleen Verhoye1, Annemie Van der Linden1

1Biomedical Sciences, Bio-Imaging Lab, University of Antwerp, Wilrijk, Belgium

The use of resting state functional MRI (rsfMRI) in preclinical research is expanding progressively, but the majority of resting state imaging is obtained in anesthetized animals. Since it is known that anesthesia may influence the functional connectivity (FC) outcome, we compared rsfMRI data from awake mice with results obtained from mice anesthetized with -chloralose, urethane or isoflurane (1%). Overall, we can conclude that to study interhemispheric FC using ICA in mice, a 15 component analysis should be used in urethane or -chloralose anesthetized animals. Moreover awake mouse rsfMRI is proven to be possible and useful but needs to be optimized.

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Keywords

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