Meeting Banner
Abstract #3078

Comparison of in vivo and Ex Vivo DTI Cortical Connectivity Measurements in the Squirrel Monkey Brain

Yurui Gao1, 2, Ann S. Choe1, 2, Xia Li1, Iwona Stepniewska3, Adam W. Anderson1, 2

1Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States; 2Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, United States; 3Department of Psychological Sciences, Vanderbilt University, Nashville, TN, United States

In a previous study, we validated DTI-tractography-derived measures of primary motor area (M1) corticocortical (CC) connectivity by comparing the ex vivo DTI connectivity with the histological ground truth. Ex vivo acquisitions typically provide higher image quality than in vivo experiments, since scan times can be much longer and motion is usually not an issue. The goal of the present study was to get a more realistic understanding of the limitations of in vivo DTI measures of CC connectivity by comparing the reliability of in vivo and ex vivo DTI data acquired from the same squirrel monkey.

Abstract PDF Presentation Video

Keywords

abbreviation acquisition acquisitions addition adjacent animal anisotropy assisted bidirectional biomedical brain coefficients columns comp comparing computer connectivity correlation correlations cortex cortical counting covering crossing decreased deformation denoted density depths derived deterministic diffusivity displays distribution dorsal engineering even every extend farther fiber fibers fields fixed forelimb fractional freezing goal gradient gray ground histological identified illustrates immediately in vivo indicated injected injection intensities interface issue light limitations live located locations longer manually maps materials matrix measures microscope monkey month morphological motion motor movement much neural normalized onto parietal passing pattern patterns photographed pipeline pixels posterior preprocessing previous primary procedure processing produced programs projected projection propagate propagated psychological quality quite rank reach reacted realistic reducing registered registration reliability rendering revealed rows sacrificed scanned scanner science sciences section sectioned seed segmentation segmented sets significantly since sites sixth space squirrel stained start step stop supplementary table tensors tracer tracking trans transferred transformed truth typically understanding validated variational white