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Abstract #2300

Effect of Four Commonly Used Anesthetics on Stimulation-Induced and Resting State fMRI Signal in Mice

Aileen Schrter1, Felix Schlegel1, Aline Seuwen1, Joanes Grandjean1, Markus Rudin1, 2

1Institute for Biomedical Engineering, ETH and University Zrich, Zrich, Switzerland; 2Institute of Pharmacology and Toxicology, University Zrich, Zrich, Switzerland

Functional MR imaging, originally applied in human studies, has been translated back to small animals, which allows performing basic research on animal models of different pathologies. In view of the many transgenic mouse lines the method has been started to be transferred from rats to mice. FMRI in small animals suffers from largely unknown influences of anesthesia, which has been addressed already in studies on rats. However, given species differences, results obtained in rat studies cannot be translated directly to mice. The presented study is characterizing the effects of four anesthetics isoflurane, urethane, medetomidine, propofol on fMRI responses in mice.

Keywords

acquisition activation advent allow amplitude analyzing anesthesia anesthetic anesthetics anesthetized animal animals applied applying array axial biggest biomedical block blocks blood bold bolus bore brain carried cascade cerebral characteristics characterize coil combination commonly comprising contra correlated correlation cryogenic cycles decay decrease delay depend dependency dependent depending depicts deviated distinction duration dynamics either electrical electrodes element except exerting experiment explaining female fixated flow function functional generating highly homogeneous horizontal increasing indicate induced induction inferences influence infusion initial institute intensity intubated involved issue larger linearly link maps metabolic mice might mixture modeling monitored mouse needle network observe obviously occurrence onset outcome oxygen particular peak period physiological placed plot polarized positive preceding preparation processing profile prominent pronounced rather readouts receive receiving recorded regime resolution response responses resting review rich room sensory significantly smaller software spatial species stimulated stimulation stimulus strength strongest strongly studies subcutaneously subsequent system technology temperature temporal translated uniformity urethane vascular ventilated