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Abstract #1950

Fast Determination of Absolute Metabolite Concentrations by Spatially-Encoded 2D NMR: Application to Breast Cancer Cell Extracts

Serge Akoka1, Adrien Le Guennec1, Illa Tea1, Estelle Martineau1, Benoit Charrier1, Meerakhan Pathan1, Patrick Giraudeau1

1Chemistry, Universit de Nantes, Nantes, France

Absolute metabolite concentrations from complex metabolic mixtures were measured using an approach based on a multi-scan single shot (M3S) strategy (derived from ultrafast 2D NMR). The analytical performance was compared to the one of conventional 2D NMR. On model metabolic mixtures, the precision was in the 1-4% range (versus 5-18% for the conventional approach), highlighting the superiority of the M3S approach for quantitative analysis. The M3S COSY approach was then applied to measure the absolute metabolite concentration of 14 major metabolites in three breast cancer cell line extracts, showing significant differences between cell lines.

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Keywords

absolute abundant acquisition addition affecting alternative analyst analytical application applied approaches assessed biological breast cancer cell chemistry coefficients complex concentration concentrations contents counterpart derived described determination determine encoded error experiment experimental explained extracts fast generally hardware human immunity increment instabilities intracellular lactate linearity measure measured metabolic metabolite metabolites minutes mixture mixtures model natl observe offers often particularly performance plotted potential powerful precision procedure prod propose protocol quantitative reasonable recording represented sample sensitivity serge series shot spatially spectra spectrometer strategies strategy subsequent superiority temporal terms tool trivial ultrafast vary whereas whose