Meeting Banner
Abstract #1710

Water Diffusion Is Disrupted in Low Collagen Containing Hypoxic Regions of Breast Cancer Xenograft

Samata Kakkad1, 2, Jiangyang Zhang3, Alireza Akhbardeh1, Meiyappan Solaiyappan1, Venu Raman1, Dieter Leibfritz2, Kristine Glunde4, Zaver M. Bhujwalla4

1JHU ICMIC Program, Division of Cancer Imaging Research, The Johns Hopkins University SOM, Baltimore, MD, United States; 2Department of Chemistry and Biology, University of Bremen, Bremen, Germany; 3Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University SOM, Baltimore, MD, United States; 4JHU ICMIC Program, Division of Cancer Imaging Research, Johns Hopkins University, Baltimore, MD, United States

Hypoxic tumor microenvironments are frequently observed in tumors and are associated with an aggressive phenotype and resistance to radiation and chemotherapy. We previously observed significantly fewer collagen fibers in hypoxic tumor regions. Diffusion tensor imaging is often used to distinguish between normal, benign and malignant breast tissue as a non-contrast imaging technique, since lower water diffusion and diffusion anisotropy parameters are observed in malignant tissue. Here we examined the relationship between low collagen containing hypoxic tumor regions and high resolution DTI and observed lower diffusion and diffusion anisotropy in hypoxic regions compared to normoxic regions within a breast cancer xenograft.

Abstract PDF

Keywords

adjustable aggressive anisotropy apparent approximately backdrop biology breast bright buffered cancer cells chaotic chemistry chemotherapy coefficient collagen color combined component confirmed consistently contain containing contribute control cover decreased delivery detect dice dieter diffusion dimensions display disrupted distributions division done drug efficient eight element engineered entire error even examined excised explain explained expressing extracellular extraction feature female fiber fibers field fixed florescence fluoresce fluorescence fluorescent fractional functional good heterogeneous human hypoxia identified immersed immunodeficient important index indicates inoculated inverted johns leads lens major mammary maps marked matrix mice microscope model molecular molecules movement optical overall overlaid overlap partridge phenotype phosphate placed plays polyether poor program protein radiation radiological radiology reason recurrence reduced register registered registration relatively reregistration resistance resolution response role saline sample science scientific section sections separate severe significantly similarity sliced slicer solution spatially spectrometer stably statistical strength studies supported tensor thick thought tissue transport tube tumor tumors volumes washed water