Meeting Banner
Abstract #1708

1H/31P Polarization Transfer at 9.4T to Detect Phosphomono- and -Diesters in Breast Tumor Models

Lu Jiang1, Jannie P. Wijnen2, Tiffany Greenwood1, W.J.M. van der Kemp2, Dennis W. J. Klomp2, Kristine Glunde3

1Division of Cancer Imaging Research, Department of Radoilogy, Johns Hopkins School of Medicine, BALTIMORE, MD, United States; 2Radiology Department, University Medical Centre Utrecht, Utrecht, Netherlands; 3Division of Cancer Imaging Research, Department of Radoilogy, Johns Hopkins University, BALTIMORE, MD, United States

The adiabatic version of refocused insensitive nuclei enhanced by polarization transfer (BINEPT) and pulse acquire (PA) 31P Magnetic Resonance Spectroscopy (MRS) are compared in MCF-7 and MDA-MB-231 breast cancer xenografts at 9.4 Tesla. After in vivo MRS measurements, the tumors were extracted and high-resolution (HR) 31P MRS was performed. Our data suggest that BINEPT can be advantageous for studying the individual compounds in phospholipid metabolism in both the choline and ethanolamine cycle, in translational research in breast cancer and other cancers in vivo at high magnetic field strength.

Abstract PDF

Keywords

accordingly accurately addition adiabatic advantageous affects aggressive another applying assessed band bottom breast built cancer cell cells chloroform clearly coil comparing concentrated constants corner corrected coupling degree depend detect detecting detection diagnosis direct division double enhanced equally even example excitation excited experiment extract extraction extracts facilitates female field fitted flat foundation free full funded furthermore greenwood grow hardly highly home hypothesis in vivo increasing individual inner inoculated insensitive intracellular intrinsically johns localized long loss macromolecules mammary measured medicine metabolism metabolite metabolites methanol mice models muscle noise nuclei nucleus nude optimal outside overview partially particularly period phosphorous polarization posses previously proliferative pulse pulses quantification quantified radiology rare refocused related relatively removing repetition reported resolution resolve schematic school segmented sensitivity seven since software solenoid soluble somewhat spectra spectrometer spectroscopy spectrum spins stage strength strengths strong studied subsequently substantial suggest taken tiffany transcript transfer translational treatment trend tumor tumors tuned useful validated version visible water width