Meeting Banner
Abstract #1704

Choline Kinase-α Protein But Not Its Activity Is Necessary in Breast Cancer Cell Proliferation

Noriko Mori1, Flonn Wildes1, Kristine Glunde2, Zaver M. Bhujwalla2

1JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States; 2JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, MD, United States

Increased levels of choline kinase (Chk)-α and phosphocholine (PC) are consistently observed in aggressive cancers. Understanding the roles of Chk and PC in cancer cells provide new insights into the malignant phenotype and can lead to the development of new treatment strategies. We previously showed that a Chk inhibitor which reduces PC had no effect on MDA-MB-231 cell proliferation, but downregulation of Chk with siRNA reduced proliferation. Here we have expanded upon these studies using SUM149 triple negative inflammatory breast cancer cells. These data confirm the importance of the enzyme, but not its activity, in breast cancer cell proliferation.

Abstract PDF

Keywords

active activity affect affects almost antibody assay assays binding breast cancer catalytic catalyzes cell cells cellular characteristics choline closely cofactor collected compound concentration conditions consistent containing contrast control culture custom days decrease decreased dependently destabilize determined development deviation done dose dual enzyme essential established examined expanded expression extraction extracts fisher form free fresh fully function grown hours human indicate inflammatory inhibiting inhibition inhibitor instruction insulin integrals internal invasion investigate ions johns lipid little loaded loading long made magnesium malignant manufacturer markedly media medicine medium metastasis metastatic molecular monoclonal necessary negative oncogene page peaks pharmaceuticals phosphate post potential previously primarily program proliferation protein quantified radiological radiology reagent reduce reduced reduces reducing reduction related relaxed representative resolved resultant role school science scientific sigma significantly site soluble spectra spectrometer spectroscopy stable starting strategies suggest supplemented support supported technologies thermo transfer transformation treated treatment triple tumors undetectable untreated vehicle vertex viability volume water whether widely