Meeting Banner
Abstract #1564

Counting Glomeruli in Mouse Kidneys Using MRI

Scott Charles Beeman1, John F. Bertram2, Jennifer R. Charlton3, Teresa Wu1, Min Zhang1, Kevin M. Bennett1

1Arizona State University, Tempe, AZ, United States; 2Monash University, Clayton, Victoria, Australia; 3University of Virginia, Charlottesville, VA, United States

Glomerular number plays an important role in a wide range of renal and systemic diseases, though the techniques currently employed to count glomeruli are estimates extrapolated from a small sample. A method for counting every glomerulus in the kidney would provide information never before available. Here, we demonstrate the first steps taken towards visualizing and counting all glomeruli in the mouse kidney. With the huge library of transgenic mouse models employed in kidney research, such a technique may prove vital to basic kidney research.

Keywords

according accumulation accurate animal another application basement best binding blood capable cationic caused cell charge charged clear clinical comparable components conducted conjugating considered control cortex count counted counting counts coupled custom dark darkening defined develop diagnostics dial dimethyl directly discovery disease distinguish doses dramatic drug employed endowment estimating ethyl every extract extrapolates false filters find function functioning future goal gradient gradients guided histology horse hours huge hypertension identified in vivo individual injected injection injections intravenous iron kidney kidneys knowledge labeled library light linked located major male many measure membrane mice microscopes might models morphological mouse naive noted occur organ original outfitted overlapping perfused plasma population positives possibility post preparation processing produce profound rats regional regulates renal report represents resolution revealed sacrificed sample sampling sections serial serve sigma software spatial spleen spot spots studies suggest synthesis system systematic systemic taken thereby throughout tissue tool toxicity useful validate versus volume volumes watershed wide yielded yields