Meeting Banner
Abstract #1268

MEMRI Based NOD/scid-IL-2R&[gamma] cnull Mouse Brain Atlas for HIV Pathobiology Studies

Balasrinivasa R. Sajja1, Biyun Zhou2, Mariano G. Uberti1, Larisa Y. Poluektova3, Howard E. Gendelman3, Michael Douglas Boska1, 3, Yutong Liu1, 3

1Radiology, University of Nebraska Medical Center, Omaha, NE, United States; 2Anesthesiology, Tongji Medical College, Huanzhong University of Science and Technology, Wuhan, China; 3Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, United States

Strain specific mouse brain MR atlases provide a coordinate space for spatial normalization and permit longitudinal quantitative analyses associated with aging and disease progression. Manganese enhanced MRI (MEMRI) enhances anatomic structures in the NOD/scid-IL-2R&[gamma] cnull (NSG) mice. NSG mice can propagate human cell transplants and thus be used to study human disease. Image post-processing based on Laplacian operator was used to enhance brain structures boundaries without introducing artifacts. Thus, an in vivo MEMRI based NSG mouse brain atlas was generated. This atlas will be used to track structure-wise alterations during progression of HIV-1 in NSG mice implanted with human immune cells.

Abstract PDF

Keywords

abilities ability accept accurate acquisition administration affine aging aligned allow alterations analyze anatomic anesthetized animal applied applying assessments atlas atlases axial blood body boundaries brain brains breaths breeding built cell cells china chosen coil college colony column columns combined coronal corrected create created date deformation determine disease enhanced enhancement errors evaluations experimental extended features final finally fourth generated grams hours human identification immune implemented improved improvement in vivo individual infection inhalation inhomogeneity institution intensity interpolation isotropic iterations iteratively label labeled labeling laboratory longitudinal maintained major male manganese manually mapping matrices matrix median medical metric mice middle minimize minute morphological mouse nineteen nonlinear operator oxygen pharmacology pixel preliminary preparation procedures progression project radiology reflect registered registration rendering represent representative represents require resonator respectively rigid scanner science separating sharpen sharpness sled slices software step strains structure structures studies substructures successfully surface technology third tissue track trans transplanted transplants trends updated visible volume volumes wise year