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Abstract #1053

Increased Hippocampal Glutamate After Sleep Deprivation in the Pre-Pubescent BALB/cJ Mice: An in-vivo 1H MRS Study

Manoj Kumar1, Gaurav Verma1, Ranjit Ittyerah1, Stephen B. Pickup1, Edward S. Brodkin2, Ted Abel3, Harish Poptani1

1Radiology, University of Pennsylvania, Philadelphia, PA, United States; 2Psychiatry, University of Pennsylvania, Philadelphia, PA, United States; 3Biology, University of Pennsylvania, Philadelphia, PA, United States

In-vivo spectroscopy was performed after 3 hours of sleep deprivation in the less social BALB/cJ and more social C57BL/6J mice. Significantly increased glutamate was noted in sleep deprived BALB/cJ as compared to control non-sleep deprived animals. The more social C57BL/6J mice did not exhibit this behavior. An abnormal increase in electrical activity resulting from excessive glutamate signaling causes prolonged alterations in behavior, as commonly seen in autism. Abnormalities in glutamatergic neurotransmitter system may partially underpin the pathophysiology of autistic spectrum disorders and hence glutamate and its regulatory molecules are considered as potential targets for these disorders.Non-invasive MRS methods may be useful in assessing these metabolic alterations.

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Keywords

acid acquisition activity adjusted analyzed anesthesia animal animals arbitrary arrow awake behavior behavioral biology birdcage blowing body bore brain cages caused clearly coil complex compute concentration concentrations connected considered control controls core demonstrating deprivation deprived developed developmental device directly disease disorders dissolved duration efficient electrodes excitatory extracted extraction extracts frozen functions funded future glutamate grants harvesting healthy home homeostatic house immediately impaired important in vivo included induced instruments internal learning liquid location magnet maintained maintaining major matched materials measure memory metabolites mice minimize model monitor motion motor mouse neuropsychiatric neurotransmitter neurotransmitters nose overdose part pharmacological physiological pickup placed placing planning potential preparation previous progression psychiatry pubescent pulse radiology receive reduced regulatory relationship reported resolution resolved respiration respiratory restraining sacrificed samples scanner schizophrenia separately significantly sleep sociability social spectra spectral spectroscopic spectroscopy spectrum spin stored stroking studies susceptible symptomatology system systems table taken targets temperature therapeutic tissue treatment undisturbed vertical vital vitro warm water