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Abstract #0863

Oxygen-17 MRS for CMRO2 Measurements in the Mouse Brain at 16.4T

Weina Cui1, Xiao-Hong Zhu1, Manda Vollmers1, Emily Colonna1, Gregor Adriany1, Brandon Tramm2, Janet Dubinsky1, Gulin Oz1

1University of Minnesota, Minneapolis, MN, United States; 2Virtumed, LLC, Minneapolis, MN, United States

To assess cerebral energetics in transgenic mouse models of neurological disease, a robust and quick method for quantification of cerebral oxygen consumption is needed. 17O MRS methodology has been validated in rats and cats to measure CMRO2, however mice present unique challenges due to their small size. We show that 17O MRS in the mouse brain is feasible with high sensitivity using 16.4T, a surface 1H/17O coil and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.

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Keywords

abundance accurate acquisition administered agreement anesthetized applications assess assuming background bench bore brain built cats cerebral challenges challenging coil consumption controlled course critically crosstalk dead delivered delivery designed detection discovery disease either energetics energy ensure essential failure fashion feasibility feasible field fields finally function fund glucose good healthy home hong in vivo incorporation increasing index inhaled inside intubation investigated investigations involvement labeled leak linear living localized maps measure measured metabolic metabolism methodology mice minimal minimization minutes mixture modeling models modified mouse natural need needed neurological newly next numerous oral oxygen physiology potential produces pulse quadrature quantification quick quickly rapid rapidly rats regular reliable reliably repeatedly reported required reservoir robust scanner sensitivity slope space spectra studied substantially sufficient summary supported supra surface switch switched switching system thereby ultra unique utilization utilized utilizing validated