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Abstract #0808

Mutation in Isocitrate Dehydrogenase 1 (IDH1) Leads to Increased T2, ADC and Decreased Lactate and Glutamate in Glioblastoma Model

Jelena Lazovic1, Soto Horacio2, Sichen Li3, Albert Lai3, Linda Liau2, Robert Prins2, Timothy F. Cloughesy4, Whitney Pope5

1Radiology, University of California at Los Angeles, Los Angeles, CA, United States; 2Neurosurgery, University of California, Los Angeles, CA, United States; 3Neuro-Oncology, University of California, Los Angeles, CA, United States; 4Neuro-Oncology, UCLA, Los Angeles, CA, United States; 5Radiology, University of California, Los Angeles, CA, United States

Recent reports strongly correlate presence of isocitrate dehydrogenase 1 (IDH1) mutation (IDH1-R132) with improved overall survival among glioblastoma patients. This mutation is associated with production of 2-hydroxyglutaric (2-HG) acid, with not well established role in malignant progression. Human glioblastoma cell line (U87) was modified to overexpress mutated isocitrate dehydrogenase 1 (IDH1) R132 in order to determine if 2-hydroxyglutaric acid could be detected in vivo and the consequence on tumor growth and metabolism. We found glioblastoma that overexpressed IDH1-R132 to have significantly increased growth rate along with accumulation of 2-HG and reduction in lactate and glutamate.

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Keywords

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