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Abstract #0673

Impact of Isocaloric Fructose and Glucose Diets on Lipid Metabolism Studied in Vivo by Multinuclear MR Spectroscopy

Patricia M. Nunes1, Alan J. Wright1, Andor Veltien1, Jack JA van Asten1, Cees J. Tack2, John G. Jones3, Arend Heerschap1

1Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Gelderland, Netherlands; 2Internal Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, Gelderland, Netherlands; 3Center for Neurosciences and Cell Biology, Coimbra, Portugal

We investigated in vivo the effects of isocaloric fructose and glucose feeding of mice over 8 weeks on ectopic lipid deposition and hepatic energy status by MRS. Hepatic lipid sources were resolved into dietary, which was investigated in vivo with 1H{13C}MRS after an oral bolus of [U-13C]lipids, and de novo lipogenesis which was assessed by 2H NMR spectroscopy using 2H2O as tracer. Isocaloric fructose feeding had no effect on intramyocellular lipids, but significantly increased hepatic lipids, most likely by stimulating de novo lipogenesis and not by promoting dietary fat uptake. This occurs without expense of net hepatic energy, as shown by 31P MRS, unlike acute doses of fructose.

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